ST 3072
Description
Identification of bacterial strains at genus or species level by 16S rRNA gene sequencing.
Scope
Genus- or species-level identification by PCR, sequencing and database comparison.
Methodology
Cell lysis by high temperature and/or Chelex-100 resin. PCR amplification (primers fD1/rD1 or Y1/Y2). Sequencing and database comparison for taxonomic identification.
Application field
Terrestrial environment
Keywords
Sequencing – 16S rRNA gene – Bacterial identification
Responsible persons (source)
- Antonio Lagares