ST 3073
Description
Microbial identification complementary to MALDI-TOF by amplification of species-specific genomic regions.
Scope
Complementary to MALDI-TOF; amplification of species-specific genomic regions and gel electrophoresis detection.
Methodology
Cell lysis, DNA extraction, PCR with custom primers. Detection by agarose gel electrophoresis and UV visualization.
Application field
Terrestrial environment
Keywords
PCR – Primer design – Microbial identification
Responsible persons (source)
- Antonio Lagares